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《医学前沿(英文)》 2021年 第15卷 第3期 页码 438-447 doi: 10.1007/s11684-020-0826-1
关键词: sporadic thoracic aortic dissection exome sequencing gene COL3A1 case–control study extracellular matrix
Novel variants in LAMA3 and COL7A1 and recurrent variant in KRT5 underlying epidermolysis bullosa in
《医学前沿(英文)》 2022年 第16卷 第5期 页码 808-814 doi: 10.1007/s11684-021-0878-x
关键词: epidermolysis bullosa LAMA3 COL7A1 KRT5 Chinese families
Influence and related mechanism of Retn gene expression on glucose uptake in 3T3-L1 cells
LI Yahui, DONG Shiyuan, YU Chao, JIANG Yu, LI Huaixing, SUN Shuhan
《医学前沿(英文)》 2007年 第1卷 第3期 页码 269-273 doi: 10.1007/s11684-007-0051-1
关键词: 3T3-L1 influence resistance receptor substrate-1 transport
APPLE SUMO E3 LIGASE MDSIZ1 NEGATIVELY REGULATES DROUGHT TOLERANCE
《农业科学与工程前沿(英文)》 2021年 第8卷 第2期
Drought stress typically causes heavy losses in apple production and uncovering the mechanisms by which apple tolerates drought stress is important in apple breeding. MdSIZ1 is a SUMO (small ubiquitin-like modifier) E3 ligase that promotes SUMO binding to substrate proteins. Here, we demonstrate that MdSIZ1 in apple has a negative relationship with drought tolerance. MdSIZ1 RNAi transgenic apple trees had a higher survival rate after drought stress. During drought stress they had higher leaf water potential, reduced ion leakage, lower H2O2 and malondialdehyde contents, and higher catalase activity. In addition, MdSIZ1 RNAi transgenic plants had a higher net photosynthetic rate during the latter period of drought stress. Finally, the transgenic apple trees also altered expression levels of some microRNAs in response to drought stress. Taken together, these results indicate that apple MdSIZ1 negatively regulates drought stress by enhancing leaf water-holding capacity and antioxidant enzyme activity.
APPLE SUMO E3 LIGASE MDSIZ1 NEGATIVELY REGULATES DROUGHT TOLERANCE
Baohua CHU, Jia SUN, Huan DANG, Ziqing MA, Shuang ZHAO, Qingmei GUAN, Xuewei LI
《农业科学与工程前沿(英文)》 页码 247-261 doi: 10.15302/J-FASE-2021388
Bioinformatic exploration of MTA1-regulated gene networks in colon cancer
null
《医学前沿(英文)》 2016年 第10卷 第2期 页码 178-182 doi: 10.1007/s11684-016-0442-2
Metastasis-associated gene 1 (MTA1) controls a series of biological processes in tumor progression. Tumor progression is a complex process regulated by a gene network. The global cancer gene regulatory network must be analyzed to determine the position of MTA1 in the molecular network and its cooperative genes by further exploring the biological functions of this gene. We used TCGA data sets and GeneCards database to screen MTA1-related genes. GO and KEGG pathway analyses were conducted with DAVID and gene network analysis via STRING and Cytoscape. Results showed that in the development of colon cancer, MTA1 is linked to certain signal pathways, such as Wnt/Notch/nucleotide excision repair pathways. The findings also suggested that MTA1 demonstrates the closest relationship in a coregulation process with the key molecules AKT1, EP300, CREBBP, SMARCA4, RHOA, and CAD. These results lead MTA1 exploration to an in-depth investigation in different directions, such as Wnt, Notch, and DNA repair.
关键词: metastasis-associated gene 1 colon cancer bioinformatics
NES1/KLK10 and hNIS gene therapy enhanced iodine-131 internal radiation in PC3 proliferation
Jiajia Hu, Wenbin Shen, Qian Qu, Xiaochun Fei, Ying Miao, Xinyun Huang, Jiajun Liu, Yingli Wu, Biao Li
《医学前沿(英文)》 2019年 第13卷 第6期 页码 646-657 doi: 10.1007/s11684-018-0643-y
关键词: androgen-independent prostate cancer normal epithelial cell-specific 1/kallikrein 10 sodium/iodide symporter radiation therapy proliferation
Qiong CHEN, Qing YU, Yuhu SONG, Peiyuan Li, Ying CHANG, Zhijun WANG, Lifeng LIU, Wei WU, Jusheng LIN
《医学前沿(英文)》 2009年 第3卷 第2期 页码 148-152 doi: 10.1007/s11684-009-0032-7
关键词: gene X-linked inhibitor of apoptosis protein associated factor-1 (XAF1) promoter transcription regulation
《医学前沿(英文)》 2022年 第16卷 第4期 页码 627-636 doi: 10.1007/s11684-020-0815-4
关键词: RUNX1 gene mutation acute myeloid leukemia transcriptional repression DNA methylation
Identification of an E3 ligase-encoding gene
Yanfei Zhang, Xinchun Zhao, Yongchun Zhou, Min Wang, Guangbiao Zhou
《医学前沿(英文)》 2020年 第14卷 第3期 页码 318-326 doi: 10.1007/s11684-019-0708-6
关键词: RFWD3 NSCLC prognosis tobacco smoke
Antitumor immunity of human SART3 gene vaccine against mouse tumor
HE Yu, YANG Shuhua, LIU Yong, LI Tao
《医学前沿(英文)》 2008年 第2卷 第1期 页码 51-57 doi: 10.1007/s11684-008-0010-5
关键词: antitumor therapy occurrence implantation DNA vaccine SART3 DNA
Xudong YU MM, Zengwu SHAO MD, Liming XIONG MD, Weiwei XU MM, Hezhong WANG MM, Huifa XU MM,
《医学前沿(英文)》 2009年 第3卷 第4期 页码 415-420 doi: 10.1007/s11684-009-0072-z
关键词: tissue inhibitor of metalloproteinase-3 intervertebral disc rabbit gene therapy
《医学前沿(英文)》 doi: 10.1007/s11684-023-1003-0
关键词: Lipin3 Lipin1 hypertriglyceridemia obesity mitochondrial dysfunction
Gene therapy for hemophilia B mice with scAAV8-LP1-hFIX
null
《医学前沿(英文)》 2016年 第10卷 第2期 页码 212-218 doi: 10.1007/s11684-016-0438-y
Hemophilia B is a hemorrhagic disease caused by the deficiency of clotting factor IX (FIX). Gene therapy might be the ultimate strategy for the disease. However, two main problems that should be solved in gene therapy for hemophilia B are immunity and safety. Self-complementary adeno-associated virus serotype 8 (scAAV8), a non-human primate AAV featuring low immunogenicity and high transfection efficiency in liver cells, might be a potential vector for hemophilia B gene therapy. A strong liver-specific promoter-1 (LP1) was inserted and mutant human FIX Arg338Ala was introduced into plasmid scAAV8-LP1 to develop an optimized AAV8 vector that expresses human clotting factor FIX (hFIX). The efficiency of scAAV8-LP1-hFIX administered through normal systemic injection or hydrodynamic injection was compared. A high expression was achieved using hydrodynamic injection, and the peak hFIX expression levels in the 5×1011 and 1×1011 virus genome (vg) cohorts were 31.94% and 25.02% of normal level, respectively, at 60 days post-injection. From the perspective of long-term (200 days) expression, both injection methods presented promising results with the concentration value maintained above 4% of normal plasma. The results were further verified by enzyme-linked immunosorbent assay and activated partial thromboplastin time. Our study provides a potential gene therapy method for hemophilia B.
关键词: hemophilia B AAV8 hFIX gene therapy
null
《医学前沿(英文)》 2014年 第8卷 第2期 页码 217-226 doi: 10.1007/s11684-014-0326-2
This study was designed to investigate the contribution of miRNA-122-binding site polymorphism at the IL-1A gene and its multiplicative interactions with hepatitis B virus (HBV) mutations in the risk of hepatocellular carcinoma (HCC). A total of 1021 healthy controls, 302 HBV surface antigen (HBsAg) seroclearance subjects, and 2011 HBsAg-positive subjects (including 1021 HCC patients) were enrolled in this study. Quantitative PCR was used to genotype rs3783553. HBV mutations were determined by direct sequencing. Multivariate logistic regression analyses were performed to test the associations of rs3783553, mutations, and their interactions with the risk of HCC. No significant association was found between rs3783553 and the risk of HCC among healthy controls, HBsAg seroclearance subjects, HBsAg-positive subjects without HCC, and all controls. Additionally, rs3783553 was not significantly associated with chronic HBV infection, liver cirrhosis, HBV e antigen seroconversion, abnormal alanine aminotransferase, and high viral load (>104 copies/ml). However, the TTCA insertion allele of rs3783553 was significantly associated with an increased frequency of HBV C7A mutation compared with homozygous TTCA deletion carriers [(del/ins+ ins/ins) vs. del/del, adjusted odds ratio (OR)=1.48, 95% confidence interval (CI)=1.09-2.02, P=0.013]. Multiplicative interaction of rs3783553 with HBV preS deletion significantly reduced the risk of HCC in males, with an adjusted OR of 0.64 (95% CI=0.42-0.98; P=0.041) after age and HBV genotype were adjusted. Although rs3783553 did not significantly affect genetic susceptibility to HBV-related HCC, its variant allele may predispose the host to selecting HBV C7A mutation during evolution and significantly reduce the risk of HCC caused by HBV preS deletion. This study provides an insight into the complex host-virus interaction in HBV-induced hepatocarcinogenesis and is helpful in determining HBsAg-positive subjects who are likely to develop HCC.
关键词: hepatocellular carcinoma (HCC) interaction miRNA-122-binding site IL-1A rs3783553 hepatitis B virus (HBV) mutations
标题 作者 时间 类型 操作
Identification of variants associated with sporadic thoracic aortic dissection: a case--control study
期刊论文
Novel variants in LAMA3 and COL7A1 and recurrent variant in KRT5 underlying epidermolysis bullosa in
期刊论文
Influence and related mechanism of Retn gene expression on glucose uptake in 3T3-L1 cells
LI Yahui, DONG Shiyuan, YU Chao, JIANG Yu, LI Huaixing, SUN Shuhan
期刊论文
APPLE SUMO E3 LIGASE MDSIZ1 NEGATIVELY REGULATES DROUGHT TOLERANCE
Baohua CHU, Jia SUN, Huan DANG, Ziqing MA, Shuang ZHAO, Qingmei GUAN, Xuewei LI
期刊论文
NES1/KLK10 and hNIS gene therapy enhanced iodine-131 internal radiation in PC3 proliferation
Jiajia Hu, Wenbin Shen, Qian Qu, Xiaochun Fei, Ying Miao, Xinyun Huang, Jiajun Liu, Yingli Wu, Biao Li
期刊论文
Cloning of human XAF1 gene promoter and assay of its transcription activity in a variety of cell lines
Qiong CHEN, Qing YU, Yuhu SONG, Peiyuan Li, Ying CHANG, Zhijun WANG, Lifeng LIU, Wei WU, Jusheng LIN
期刊论文
Distinct gene expression pattern of mutations coordinated by target repression and promoter hypermethylation
期刊论文
Identification of an E3 ligase-encoding gene
Yanfei Zhang, Xinchun Zhao, Yongchun Zhou, Min Wang, Guangbiao Zhou
期刊论文
Antitumor immunity of human SART3 gene vaccine against mouse tumor
HE Yu, YANG Shuhua, LIU Yong, LI Tao
期刊论文
Adenovirus-mediated tissue inhibitor of metalloproteinase-3 gene transfection inhibits rabbit intervertebral
Xudong YU MM, Zengwu SHAO MD, Liming XIONG MD, Weiwei XU MM, Hezhong WANG MM, Huifa XU MM,
期刊论文
Haploinsufficiency of Lipin3 leads to hypertriglyceridemia and obesity by disrupting the expression andhypertriglyceridemia and obesity by disrupting the expression and nucleocytoplasmic localization of Lipin1
期刊论文